Organic compounds having particular heterocyclic ring system have wide range of applications and used in pharmaceuticals, agrochemicals, veterinary products and for several other applications. Azaindoles have received considerable interest in view of their potential relationship to pharmacologically important indoles and purine nucleus, because of their interesting biological activities. Hence a new azaisatins derivative containing 2-phenylquinazolin-4(3H)- ones has been designed and synthesized. Their structures have been elucidated on the basis of elemental analysis and spectral studies. All the compounds synthesized were screened for their potential antimicrobial activities, which exhibited some authentic results towards testing organism invitro and invivo studies.