Reach Us 44-7452-259145


Simultaneous determination of Vincristine and Vinblastine in Vinca rosea leaves by High Performance Thin Layer Chromatography

A simple, sensitive and specific thin layer chromatography densitometric method has been developed for the simultaneous quantification of vincristine and vinblastine in the leaves of catharanthus roseus. The method involved simultaneous estimation of vincristine and vinblastine after resolving it by High Performance TLC on silica gel plate with toluene-methanol-diethylamine (8.75: 0.75: 0.5 v/v/v) as the mobile phase. The method was validated as per the ICH guidelines for precision (inter-day, intra-day, inter-system), robustness, accuracy, LOD and LOQ. The relationship between the concentration of standard solutions and the peak response was linear within the concentration range of 100ng/spot to 4000ng/spot for vincristine and 200ng/spot to 4000ng/spot for vinblastine. The method precision was found to be 0.77-1.78 (%RSD) and 1.24-2.13 (% RSD) for vincristine and vinblastine, respectively. Accuracy of the method was checked by recovery study conducted at three different levels and the average percentage recovery was found to be 100.21 % for vincristine and 99.99 % for vinblastine, respectively. The HPTLC method for the simultaneous quantification of vincristine and vinblastine was found to be simple, precise, specific, sensitive and accurate and can be used for routine analysis and quality control of raw material of C. roseus. and several unani and ayurvedic formulations containing as an ingredient.

Author(s): Kamal Abid, Ahmad Sayee, Ahmad Farhan Jalees, Saleem Kishwar

Abstract | Full-Text | PDF

Share this  Facebook  Twitter  LinkedIn  Google+
30+ Million Readerbase
Recommended Conferences
Google scholar citation report
Citations : 7538

International Journal of Drug Development and Research received 7538 citations as per google scholar report

International Journal of Drug Development and Research peer review process verified at publons
Abstracted/Indexed in
  • Google Scholar
  • Genamics JournalSeek
  • China National Knowledge Infrastructure (CNKI)
  • CiteFactor
  • Scimago
  • Directory of Research Journal Indexing (DRJI)
  • WorldCat
  • Publons
  • MIAR
  • ResearchGate
  • University Grants Commission
  • Secret Search Engine Labs
  • Euro Pub

View More »

Flyer image


tempobet giriş

tempobet giriş

tipobet süpertotobet yeni adres süperbahis 747 güvenilir bahis siteleri telefonda sex sohbet

rolex replica