The ethanolic extract of leaves of Holoptelea integrifolia traditionally used in Indian system of medicine and exhibit wide range of biological activity. Plant extract have evolved as a source of bioactive compounds and possess a potential for developing novel therapeutic agents. All these biological activity are subjected to further studies aimed to develop promising bioactive compound for preclinical and clinical trials. According to ethno-medical studies leaves and stem bark of this plant have been reported for antiviral, antioxidant, antimicrobial, abortifacient activity and used in management of cancer. The aim of the present study was to evaluate the analytical and medicinal property of the leaves extract of Holoptelea integrifolia.