UV Spectrophotometric methods for estimation of Ramipril in Pharmaceutical dosage form by absorption maxima method and area under curve

A new, simple, rapid and novel spectrophotometric method has been developed for estimation of Ramipril (RAM). For this Absorption maximum Method (method A) and Area under Curve Method (Method B) is used. The method involved measurement of absorbance at wavelengths 210 nm for method A and method B involved measurement of area under curve in the wavelength range 202 to 237.5 nm for RAM. Beer’s law obeyed in concentration range of 0.1 to 3.5 μg/ mL by both the methods. The proposed methods are recommended for routine analysis since they are rapid, simple, accurate and also sensitive and specific. The results obtained are reproducible with a coefficient of variation less than 2%. These methods were validated for precision, reproducibility, linearity and accuracy as per ICH guidelines.

Author(s): Syed Iftequar ,Lahoti Swaroop , Zahid Zaheer ,Mirza Shahid, Sayad Imran, M H Dehghan

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