As a part of research project on the synthesis of number of substituted benzotriazole derivatives with electron donating as well as electron withdrawing groups was done and evaluated them for antibacterial and antifungal activity. First of all benzotriazole was prepared using o-phenylene diamine and sodium nitrite in acidic conditions. Now benzotriazole was reacted with ethyl chloroacetate to form benzotriazole ethyl acetate which was then reacted with hydrazine hydrate to produce benzotriazole acetohydrazide. Finally it was reacted with different sulfonyl chlorides and benzoyl chlorides to give various derivatives. The purity of all compounds have been checked by the TLC monitoring and the confirmation of the structure is checked by different spectral analysis like UV, IR, Mass and NMR and evaluated as antibacterial agent by using sulfacetamide as standard drug and for antifungal activity by using clotrimazole as standard drug.