Statistical analysis on optimisation of Microbial Keratinase enzyme screened from Tirumala and Tirupati soil samples

Due to the availability of powerful expression systems in various microbes, the large scale commercial production of new and useful enzymes is becoming increasingly attractive (10.11), The development of microbial enzymes for commercial use is a specialized field which requires.Continuous screening of new and improved strains from potential sources.Scaling up of enzyme production by optimizing conditions of fermentation(1,2,3,4,12,14) .Strain improvement of the isolated strains by classics or by recombinant methods of genetic manipulation. Statistical analysis, Down stream purification technology and formulation of enzymes(12,13,15). The biochemical heterogenicity and ecological diversity of microbes including actinomycetes and their exceptional capacity of producing secondary metabolites make them an obvious target as the source of new enzymes. They are considered potential sources of proteases with typical substrate specificities such as keratinases which attack the normally unreactive keratin(7,8). Microbial keratinases play a key role in leather processing, (5),bioconversion of keratinous waste materials to feed supplement, or source of amino acids. Thus keratinase producing organisms have both economic and ecological value (6,7,8,14). Studies on statistical analysis of keratinase have not been fully exploited hence it is addressed as the primary objective of the present study.

Author(s): Suneetha Vuppu and Raj Vuppu

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