Nano science is the basic Study of systems and materials at the nanoscale. The objective of the present study focuses on the Phytoconstituent mediated biological synthesis of ZnO-NPs by Zinc acetate and sodium hydroxide by utilizing the biocomponents of leaves of Adhatoda vasica .The samples are characterized by UV-Vis Spectroscopy, Scanning Electron Microscopy (SEM), Energy Dispersive Xray (EDAX), X-ray diffraction (XRD) and FT-Raman Spectroscopy. The Synthesized ZnO-NPs were found to be discoid in Shape with an average size of 19 - 60 nm .The diffraction pattern also confirmed that the higher percentage of ZnO with fine particle size. Phytochemicals present in the plant were responsible for the quick reduction of Zn+ ion to metallic Zinc Oxide nanoparticles. The synthesized ZnO-NPs had the potential to mitigate the bacterial cell proliferation particularly Escherichia coli, Bacillus thuringiensis, Pseudomonas aeurogonisa and Staphylococcus aureus. Adhatoda vasica is a good source for rapid reduction of metallic Zinc oxide in to nanoparticles with antibacterial activity. These green synthesized ZnO-NPs are possess therapeutic values.