In the present study, sequential extracts of Camellia sinensis, Glycyrrhiza glabra, Calendula officinalis and Linum usitatissimum were analyzed for their antioxidant activity in different test systems. Phytochemical analysis indicated that amongst twelve test extracts, methanolic extract of Camellia sinensis was found to have the highest total phenolic content (104.93 ± 1.630 mg GAE /g) and FRAP value (1046.330±1.948 mg TE/g). Flavonoid content (115.503±2.984 mg RuE /g dry extract) of methanolic extract of Glycyrrhiza glabra was found to be superior among all the extracts. Highest DPPH scavenging (IC50=44.03 ±1.784 μg/ml) effect was also observed in methanolic extracts of Camellia sinensis. In vitro antimicrobial screening indicated that methanolic extracts showed promising antimicrobial activity against Staphylococcus aureus, Staphylococcus epidermidis and Propionibacterium acnes.