Dolichous biflorus Linn. Popularly known as kulthi belongs to family fabaceae. It is also known by its medicinal properties, it is one of the most effective ayurvedic medicines for treating kidney stones. Its diuretic, anthelminthic properties make it an ideal drug for urinary and digestive disorders. Pharmacognistical standardization of Dolichous biflorus seed has been carried out in the present study. The study includes macroscopic evaluation along with estimation of its physicochemical parameters, preliminary phytochemical screening, estimation of total phenolics and flavonoids as well as fingerprint profile by HPTLC, HPLC and GCMS. Determinations of contaminants such as aflatoxins and pesticides also has been analysed and found no contaminants is present in the Dolichous biflorus seed sample. The quality control parameters in present study reveals standardization profile of Dolichous biflorus which would be of immense value in botanical identification and authentication of plant drug as well as may be helpful in preventing adulteration.