Mucoadhesive polymeric (HPMC) suspension of Ofloxacin was prepared and optimised with the aim of developing an oral controlled release gastro-retentive dosage form. The qualitative analysis of the formulation was performed by FTIR, Raman Spectroscopy, XRD and SEM analyses. Ultrasonication method was used for preparation of mucoadhesive Ofloxacin suspension. FTIR (400 cm-1 to 4000 cm-1 region) and Raman (140 to 2400 cm-1 region) Spectroscopic studies were carried out and spectra were used for interpretation. X-ray powder diffraction (XRD) data of pure drug, polymer and the formulation were obtained using a powder diffractometer, scanned from a Bragg’s angle (2θ) of 10? to 70?. The dispersion of particle was observed using Scanning electron microscopy (SEM) techniques. The particle size distribution (PSD) and aspect ratio (AR) of particles in the polymeric suspension were obtained from SEM image analysis. The results from FTIR and Raman Spectroscopic analyses suggested that in formulation, the carboxylic groups of Ofloxacin and hydroxyl groups of HPMC undergo chemical interaction leading to esterification and hydrogen bonding (both intermolecular and polymeric). The XRD data suggested that the retention of crystalline nature of Ofloxacin in the formulation would lead to increase in stability and drug loading; decrease in solubility and delayed release of the drug from polymeric suspension with better bioavailability and penetration capacity. The SEM image analysis indicated that in the formulation maximum particles were having aspect ratio from 2 to 4 and standard deviation was very less, which provided supporting evidences for homogeneous, uniformly dispersed, stable controlled release Ofloxacin suspension which would be pharmaceutically acceptable.