The aim of the present study was to investigate the hepatoprotective effects of Triphala in D-Galactosamine (D-GalN) induced hepatic toxicity in mice. The mice received a single dose of galactosamine (700mg/kg, i.p) to induce hepatotoxicity; Triphala extract (1000mg/kg, i.p) and silymarin (25 mg/kg, i.p.) were administered after the injection of galactosamine. Aspartate transaminase (AST), alanine transaminase (ALT), alkaline phosphatase (ALP), Tumour necrosis factoralpha (TNF-.), bilirubin, lipid peroxidation (LPO), superoxide dismutase (SOD), catalase (CAT), glutathione peroxidise (GPx), glutathione reductase (GR), glutathione-s-transferase (GST) and total reduced glutathione were estimated in serum of the mice.It was found that D-GalN induced hepatic damage resulted in a significant (p<0.05) increase in the activity of ALT, AST, ALP, bilirubin, LPO and TNF-. level with a decrease in the levels of anti-oxidant enzymes such as SOD, CAT, GPx, GR, GST and Total reduced glutathione which attained normal levels after the treatment of Triphala extract (1000mg/kg/b.wt, i.p). These biochemical observations were supported by histopathological examination of mice liver sections. These observations demonstrate that Triphala treatment may attenuate protective activity against D-galactosamine- induced hepatotoxicity in mice.