Synthetic medicinal compounds used in the dealing of liver diseases are insufficient and can have severe undesirable effects. So there is an international trend to revisit to traditional way of treatment using plants or plant products. Several plant derived natural compounds are under research for the treatment of liver ailments. The aim of the current study was to evaluate the protective effect of rutin against carbon tetrachloride induced toxicity in Swiss albino mice. Rutin pretreatment to mice decreased the level of lipid peroxidation (LPO) significantly (p < 0.001) as compared to CCl4 treated group. Rutin also restored the activity of liver antioxidant enzymes like superoxide dismutase (SOD) and catalse (CAT), glutathione-S-transferase (GST) significantly. Histopathological examinations of liver in rutin pretreated group showed protection against CCl4 induced injuries in liver. Over all rutin is able to reverse the altered biochemical profile towards normalization due to CCl4 exposure. Our results suggest that rutin works on the liver to maintain its standard performance and reduce cell membrane instability.