The present study was carried out to evaluate phytochemical screening, characterization and In-vitro antibacterial activity of Anisomeles malabarica roots. The antibacterial activities of A.Malabarica roots were assessed by disc diffusion method against four bacterial strains S.aureus, B.subtilis, Escherichia coli and Pseudomonas aeruginosa. The preliminary phytochemical analysis of A.Malabarica roots using the solvents like n-Hexane, Ethyl acetate and Methanol revealed the presence of alkaloids, flavonoids, tannins, saponins, and glycosides. The antibacterial activities show that the methanol extracts exhibited an active antibacterial activity at 50μg/ml and produced 6.5 mm zone of inhibition against B.subtills and P.aeruginosa 7 mm zone of inhibition in the same concentration. The hexane and methanol extracts exhibit a positive antibacterial activity which produced 6 mm and 5.5 mm (both two bacterial strain) zone of inhibition. The resulting sample was analyzed by FT-IR to find out the functional group present in the extracts. The FT-IR spectrum confirmed the presence of alkanes, alcohol, ethers, carboxylic acid, amines and phenyl ring substituted the bond.