The present study was aimed to design Prednisolone conjugated-5.0G PPI dendrimer and evaluate comparative preliminary hemolytic toxicity . The hemolytic toxicity study was conducted among free 5.0G PPI dendrimer, designed Prednisolone conjugated-5.0G PPI dendrimer and PEGylated- PPI dendrimer. The degree of hemolysis was compared with the amount of released hemoglobin from RBC was estimated by UV-spectrophotometric determination at 540nm (n=3). PEGylation has been found to be suitable for modification of ethylene diamine initiator core EDA-PPI dendrimers in various research outcomes. The results of comparative hemolytic toxicity studies observed that the toxicity viz Prednisolone conjugated-5.0G PPI dendrimer is 1.42 ± 0.52, PEGylated dendrimer 2.72 ± 1.10 and free dendrimer found to be 20.39±0.82 respectively. In this study hemolytic toxicity of synthesized systems are also relatively safer and hold potential to deliver some bioactive.