Use of herbal methods for fertility regulation is widely accepted alternative for the synthetic drugs containing chemical having side effects. Ficus religiosa is a large evergreen tree found throughout India, wild as well as cultivated. It is a highly apparent plant in Ayurvedic system of medicine for the treatment of various ailments. No report is available on the antifertility activity of plant extract hence; the present study was carried out to investigate the same.The phytochemical analysis was done using GC-MS. The compound detection employed the NIST Ver. 2.0 - Year 2008 library. The results of the GC-MS analysis provide different peaks determining the presence of many phytochemical compounds with different therapeutic activities. The major phytoconstituents were n-Hexadecanoic acid; 9, 12-Octadecadienoic acid; 9, 12, 15-Octadecatrienoic acid, and Butyl 9, 12, 15-octadecatrienoate.Effects of 1% methanol extract of fruits extract was studied on the goat uterus in vitro in exposure duration dependent manner (1 hour, 4 hours and 8 hours). Effects on dimensions of uterine glands, surface epithelium, gland cell and myometrium were observed. The treatment induces a decline in uterine glands diameter which are crucial for implantation. Present observations suggest that the extract has antifertility activity and should be experimented for antifertility programme.