The phytochemical compounds and anti-ulcer activity of leaves and root extracts of Heliotropium indicum Linn. The preliminary phytochemical screening was performed by in vitro method and anti-ulcer activity was conducted by in vivo method. The phytochemical analysis revealed the presence of alkaloids, carbohydrates and glycosides, phytosterols, fixed oils and fats, phenolic compounds and tannins, flavonoids, terpenoids, proteins and amino acids. The ethanolic extract of Heliotropium indicum leaf and root was evaluated for its anti-ulcer activity against pylorous ligation induced gastric ulcer in rats. It was found that significant reduction in gastric volume, free acidity, total acidity and ulcer index. The present study concluded that the H. indicum plant extract have various bioactive compounds and anti-ulcer activity in animal models against the drug induced gastric ulcer.