Cycas beddomei Dyer, an endemic and critically endangered, tropical, dry deciduous, dioecious gymnosperm present in varied region of adjunct areas of Tirumala Hillocks, Seshachalam Biosphere Reserve, Eastern ghats, India. The objective of the study was to investigate antioxidant capacity of aqueous extracts of microsporophylls of male cones of Cycas beddomei. This study deals with the quantitative estimation of phytoconstituents viz., Total Phenolic Content (TPC), Total Flavonoid Content (TFC), Total Flavonols (TF), Total Proanthocyanidins (TPA), Extraction Yield (EY) , Quantitative analysis soluble carbohydrates, Starch, Crude fibre, Proteins, Moisture, Ash and Mineral composition of aqueous extract of male cone of cycas beddomei has been carried out. Moreover, the assessment of antioxidant capacity by standard established calorimetric methods viz., DPPH assay, TAC and ABTS assay. Entire data has evaluated statistically. Results depicted High contents of TPC, TFC, TF and TPA and exerted anti-radical aptitude. Significant correlation has been found between quantities of phyto-constituents and anti-oxidant assays. With the results, it is confirmed that the logistics of aqueous cone extract of Cycas beddomei as potential anti-oxidant by evaluating the bioavailability of phytoconstituents and provided scientific base as a valuable natural antioxidant and therapeutic agent.