The venomous property of certain fishes have been recognized for many years; however scientific investigators have begun recently in finding the pharmacological potential of their venom. A number of venomous fishes have been investigated to explore their biological activities and therapeutic potential. Venomous fish possess wide range of toxins for predation and defense. The discovery of toxins or metabolites from fish venom has been increased significantly. Scorpaenidae family includes the world’s most venomous fish species. Their venom represents an incredible source of biologically active substances which affect vital physiological function in different animal model. The venom of these fishes is diverse and chemically complex in nature. The envenomation caused by these fishes are responsible for severe pain, edema and other complications but their venom contain many pharmacologically active component. Many toxins isolated from crude venom have been reported to be affecting cardiovascular, neuromuscular systems. They are also responsible for hemolytic activities. This review describes the pharmacological potential of Scorpaenidae venom that can be useful as research tools or lead compounds for further drugs development.