The present study was carried out to set up quality control parameters for the Indigenous traditional preparation-Dhatri lauh by making the use of classical tests along with advanced analytical tools. Dhatri lauh prepared and has been characterized by determining various physicochemical parameters in comparison to marketed sample. X-ray diffraction techniques are proved to be useful in obtaining finger print profile of prepared sample. This is the first report of fingerprinting of Dhatri lauh using advanced analytical tool. This technique would be useful in studying qualitative and quantitative differences in inorganic as well as organic chemical constituents.