Burn wounds are generally prone to various types of infection. Bacterial biofilms in particular are the culprit for non-healing wound infection in burns. Most common gram-positive strains are Staphylococcus aureus and Staphylococcus species. Methicillin resistant Staphylococcus aureus (MRSA) infection is a challenging task in clinical practice. At National Burns Centre, a total of 1679 burn patients were treated within December 2015 to September 2017. Over-all 387 burn wound samples were considered for study among which 261 samples were infected and those includes 152 Pseudomonas aeruginosa, 33 Acinetobacter baumannii, 23 Kocuria kristinae, 22 Klebziella pneumoniae, 12 Candida tropicalis, 9 Methicillin resistant Staphylococcus aureus (MRSA) and 126 samples were having no infections. The bacteriostatic and bactericidal effect of various antibiotics on MRSA such as Ampicillin, Mupiocin have been shown to inhibit the bacterial protein, RNA, DNA synthesis and cell wall formation. Several biologically important chemical groups like silver nitrate solutions, povidone iodine and silver sulfadiazine have excellent in vitro antimicrobial activity. However, many of these fails to abolish infection as they get inactivated by wound exudates and bacteria like Staphylococcus aureus develop resistance (MRSA). The purpose of the study was to identify natural compounds that can be used as remedy for MRSA. Four different compounds such as Shata Dhauta Ghruta, Jatyadi Oil, Oleuropein and Ozonated Oil ware taken for demolition of MRSA growth. Experimental observation showed that only Ozonated oil showed the anti MRSA activity and antimicrobial activity for all MRSA and non-MRSA micro-organisms tested. Our results suggest that ozonated oil is a competitive antimicrobial agent that may prompt the setting up of clinical use.