Isolation and identification of Phenolic compounds by HPLC and Electrospray Ionization Mass Spectrometry of Svensonia Hyderobadensis - A Rare Medicinal Plant Taxon

The impetus for developing analytical methods for phenolic compounds in natural products has proved to be multifaceted. Hence the present study intended to isolate phenolic compounds from leaves of Svensonia hyderobadensis by using 70% acetone and poly vinyl poly pyrrolidone (PVPP); and characterized by U.V. Visible spectrometry, High performance liquid chromatography/ electrospray ionization mass spectrometry. Total 82 phenolic compounds were obtained at both positive and negative ion modes of LCMS. Among the isolated phenols, 19 phenolic compounds have been identified based on their retention time and m/z values. Namely, Caffeic acid, Rhamnetin hexoside, 1-Caffeoyl-5-feruloylquinic acid, 9-COA, Oleuropein, P-Coumaroyl hexosa, Kaempferol-3-O-glucose, Carnosic acid, Coumaroyl 5-O-caffeoyl quinic, Oleuropein aglycon, 3, 9-di-C DOA glucoside, Luteolin-3-O-glucoronide, Quercetin-3-O-glucoside, Rhamnetin hexosyl pentoside, 6-Rhamnopyranosyl oleoside, 5-pyranopelargonidin-3-glucoside, Ligstroside, 4-Malonyl-3, 5-diCQA, Rhamnetin-O-rutinoside. This study illustrate the rich array of phenolic compounds of leaves of Svensonia hyderobadensis could be utility as health beneficial bioactive compounds.


Linga Rao M, Savithramma N

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