Isolation and Identification of Antibiotic Mediated Resistant Betalactum Producing Escherichia coli from Urinary Tract Infected Patients in ErodeDistrict, Tamil Nadu, India

The objective of this study was to detect the AmpC producing organisms in the urine samples. The urinary tract infected samples were collected and processed on various agar media and identified by observing colony morphology followed biochemical tests. In 212 urine samples growth of bacteria was only seen in 63 (29.71%) samples which were identified as the Escherchia coli and 149 (70.28%) samples which found to be healthy. Antimicrobial susceptibility testing was performed using the Kirby-Bauer disk diffusion method with the following set of antibiotics: Ampicillin/ Sulbactum (10/10 µg/disc), Aztreonam (30 µg/disc), Cefepime (50 µg/disc), Cefpodoxime (10 µg/disc), Ceftazidime (30 µg/disc), Cephoxitin (30 µg/disc), Ciprofloxacin (10 µg/disc), Imipenem (10 µg/disc), Meropenem (10 µg/disc), Ofloxacin (2 µg/disc), Piperacillin/Tazobactam (100/10 µg/ disc), Tigicycline (15 µg/disc) and Pazufloxacin (5 µg) to detect extended spectrum beta lactamase on all 63 E. coli isolates. As per CLSI guidelines extended spectrum beta lactamase production was determined by the presence of augmentation towards the cephalosporins. AmpC was detected by flattening of the zone of Ampicillin/Sulbactum towards Ceftazidime. All were resistant to cefoxitin and sensitive to cefepime. AmpC beta-lactamase production was confirmed in 5 (50%) isolates of the 10 positive isolates obtained by the AmpC detection test. Hodge test was done on all AmpC producers obtained by AmpC detection test.


Tariq AL, Reyaz AL and Sathiamoorthi T

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