The present study is to investigate the antioxidant activity of ethanol extract of Spathodea campanulata leaves in different model systems. Ethanol extract of Spathodea campanulata leaves showed significant dose dependent antioxidant activity, with a direct relationship between activity and concentration of extract. The extract showed an effective free radical scavenging activity towards the nitric oxide and superoxide radicals with IC50 values of 160 and 198 µg/mL respectively. Total antioxidant capacity at 50 and 500 µg/mL of Spathodea campanulata extract was equivalent to 29.2 and 199.4 µg/mL of á-Tocopherol. At 500 µg/mL, in nitric oxide radical and superoxide radical scavenging assay it showed maximum inhibition of 81 and 79% respectively. These results clearly indicate that leaves of Spathodea campanulata is a effective antioxidant and also superoxide and nitric oxide radicals scavenging activity.