Gymnema sylvestre has been used as a traditional medicinal plant to prevent diabetes. The present study was undertaken to evaluate anthelmintic activity of hot and cold hydroalcoholic extracts of Gymnema sylvestre leaves against Pheretima posthuma. Various concentrations (25-500 mg/ml) of hot and cold hydroalcoholic extracts of Gymnema sylvestre were evaluated in the bioassay involving determination of time of paralysis (P) and time of death (D) of the worms. Albendazole was used as standard anthelmintic drug and distilled water was used as control. The results of present study indicated that the hydroalcoholic extracts significantly exhibited the paralysis in worms and also caused death of worms in dose dependent manner, among which hot maceration extract showing more significant results when compared with the cold maceration extract. Further studies have to be done to isolate the active principles responsible for the activity.