The present study was aimed to study the antibacterial and antioxidant activity of different solvent extracts viz., Methanol, Diethyl ether and aqueous of Apium graveolens seeds. Uropathogens isolated from UTI samples, were tested against 10 different antibiotics that are commercially used for the treatment. The antibiotic resistant bacteria Escherichia coli and Pseudomonas aeruginosa isolated from Urinary Tract infected patients were used to determine the inhibitory potential of A.graveolens seed extract by using agar diffusion well method. From the analysis, methanol extract was showed highest inhibition, against bacterial pathogens when compared with other solvent extracts and standard antibiotics. The antioxidant activity of A.graveolens seed extracts was carried out 2, 2-Diphenyl-1- picrylhydrazyl (DPPH) assay method. Among the different extract tested Methanol extract was showed higher antioxidant activity than that of standard Gallic acid. Therefore, A.graveolens seed extract exhibiting enormous significance in therapeutic aspects and can applied for treating the UTI.