Abstract Diabetes mellitus is the fast spreading epidemic of the 21st century and mortality rate is high among the physically inactive middle and old aged people. In the present study the impact of physical activity on the nutritional biomarkers among young, middle and older aged diabetic subjects, living in semi urban area Thanjavur town, Tamil Nadu has been investigated. For the present study, the male subjects of 35-45, 46-55 and 56-65 years were selected. Vitamin C, E, 8 carotene and folic acid analyzed as nutritional biomarkers were found to be very low in elderly diabetic persons when compared to the physically active diabetic subjects. People with diabetes had a decline in the level of nutritional biomarkers, because of increased production of free radicals in hyperglycemia. Everyday physical activity such as walking is associated with the increased insulin sensitivity, decreased oxidative stress and enhanced antioxidant defense system.