In this study, we created an experimental animal model for evaluating the kidney and heart mitochondrial Na+/K+ATPase, Ca2+ATPase and Mg2+ATPase activities on carbon tetrachloride induced oxidative stress in rats. Wistar strain male albino rats were divided into four groups. Group I animals were served as normal and Group II animals were administrated with corn oil as vehicle control. Group III was given single dose (29th day) of CCl4 in corn oil (1:1 v/v, 3ml/kg, i.p.). Groups IV was treated with ethanolic extract of Hybanthus enneaspermus. (orally at the dose of 500mg per kg body weight). In CCl4 and Hybanthus enneaspermus treated rats, the Na+/K+ATPase, Ca2+ATPase and Mg2+ATPase activities were attained normally when compared with CCl4 treated rats.