GC-MS Analysis of Phytocomponents in the Methanolic Extract of Eupatorium triplinerve

Objective: To characterize the phytochemical constituents of Eupatorium triplinerve using GC – MS. Methods: Ten grams of the powdered sample was subjected to column chromatography over silica gel (100- 200 mesh) and eluted with n-hexane, chloroform, ethanol and methanol respectively. n-hexane and chloroform did not elute much of the compounds. The methanol fraction of the Eupharbatum triplinerve was taken for GC-MS analysis. The analysis was carried out on a GC Clarus 500 GC system with a column packed with Elite – 1 (10% dimethyl poly siloxane, 30 x 0.25 mm ID x 1 EM df), the compounds are separated using with Helium as carrier gas at a constant flow 1ml/min. sample extract (2 μL) injected into the instrument was detected by Turbo gold mass detector (Perkin Elmer) with the aid of the Turbo mass 5.1 software. Results: The GC MS analysis provided peaks of eleven different phytochemical compounds namely hexadecanoic acid (14.65%), 2,6,10-trimethyl,14-ethylene-14-pentadecne (9.84%), Bicyclo[4.1.0]heptane, 7-butyl- (2.38%), Decanoic acid, 8-methyl-, methyl ester (3.86%), 1-undecanol (7.82%), 1-hexyl-1-nitrocyclohexane (2.09%), 1,14-tetradecanediol (6.78%), Octadecanoic acid, 2-hydroxy-1,3-propanediyl ester (19.18%) and 2-hydroxy-3-[(9E) -9-octadecenoyloxy] propyl(9E)-9-octadecenoate (8.79%). Conclusion: The bioactive compounds in the methanolic extract of Eupatorium triplinerve have been screened using this analysis. Isolation of individual components would however, help to find new drugs.

Author(s): Christy Selvamangai, Anusha Bhaskar

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