Ornidazole is used for the management of amoebiasis. The site of absorption of Ornidazole is in the whole GI tract and has a long half life of 13 h. The aim of the study was to develop colon targeted film coated tablets of Ornidazole using HPMC K100, Eudragit S 100, and Ethyl cellulose as carriers in the treatment of amoebiasis. All the formulations (F1 to F8) were evaluated for the physicochemical parameters and were subjected to in vitro drug release studies. The amount of Ornidazole released from tablets at different time intervals was estimated by UV spectrophotometer. The formulation F7 released 99.38 % of Ornidazole. The results of the study showed that formulation F7 is most likely to provide targeting of Ornidazole for local action in the colon owing to its minimal release of the drug in the first 5 h. The most satisfactory formulation was stable during stability studies conducted for 60 days as per ICH guidelines. It showed no significant changes in the physicochemical parameters, in vitro release pattern.