A total of four experiments were carried out at different doses (100, 200 and 400 mg/kg, p.o.) of Dhatri Lauha in different animal model in an attempt to confirm the safety of the general patients or users of the society and country as a whole. These were Hole cross test, Hole board test, Open field test and Climbing out test. In Hole cross test highly significant (p<0.005) increase in motor activity was observed but only at a dose of 200mg/kg and only at min 30. Highly significant (p<0.005) increase in ambulation behavior was observed in Hole board test only at a dose of 100mg/kg after 240 min and a significant increase (p<0.01) was observed at the same dose after 180 min. No statistically significant changes were observed in case of head-dipping and emotional defecation in the Hole board test. Open field test showed no significant changes in ambulation, center ambulation, standing up behavior and emotional defecation. Climbing out test also did not produce any significant changes in activity. Overall the study reflects inconsistent and insignificant changes in behavior related to CNS activities which clearly demonstrates the safety of Dhatri Lauha.