Now day scientists focus the research to develop the novel cough suppressant therapies of herbomineral formulation because in respiratory disease cough is an important defensive pulmonary reflex. It removes fluids, irritants, or foreign substances. When cough becomes non-productive and require suppression and opioid receptor agonists which do not have respiratory suppressant activity but opoids produce side effects such as sedation, addiction potential and constipation, and also compromise the respiratory function. The present study was carried out to evaluate the antitussive activity of polyherbomineral formulation on cough reflex induced by different cough induced models in mice. Healthy albino mice of either sex, weighing 25-30 g were divided into seven groups, (n = 6). Group I considered as control, Group II and III received lab prepared herbomineral formulation (LPHF) (250 and 500 mg/kg, p.o.), Group VI and VII treated with marketed formulation (MF) (250 and 500 mg/kg, p.o.), Group IV and V were positive control and treated with standard (10 and 20 mg/kg, p.o.) at a dose of 0.3 ml/mice, orally. Antitussive activity of LPHF and MF were studied by sulphur dioxide gas and Ammonium liquor induced cough in mice. All the formulations used showed significant antitussive activity in sulphur dioxide induced cough model. Thus, these formulations can prove to be useful for alleviating cough. Therefore, the poly herbomineral formulation contains a major piperine active constituent which induces a cough suppressant pharmacological effect and represents an attractive approach in phytotherapeutic managements.