AIM: To investigate the antimicrobial, antioxidant and wound healing activities of different fractions of the methanolic extract of the leaves of Nerium Oleander Linn . METHODS: Antimicrobial activities were carried out by the minimum inhibitory concentration (MIC) through disc diffusion methods and MBC, the wound healing studies were carried out using ether anaesthetized rats in two different wound models Incision and Excision at two different concentrations(5% and 10%w/w). The free radical scavenging activity was studied in vitro by measuring DPPH, Hydrogen peroxide scavengering activity, Superoxide free radical (O2) and Nitric oxide (NO) free radical scavenging activity with reference to standard antioxidant ascorbic acid. RESULTS: The test compounds like crude methanolic extract (both concentrations) and different fractions like chloroform, methanol and ethyl acetate Significantly increase in tensile strength and the rate of wound contraction compared to the control and nitrofurazone. Antimicrobial activities were evaluated against seven microorganisms namely. E. faecalis, S. aureus, A. baumannii, E. coli, P.merabilis, P.aeruginosa, showed significant activity with MIC and MBC respectively. Different fungal strains like C.albicans and A.niger are not inhibited by different test compounds. The different extracts and fractions of these plants showed remarkable antioxidant activity. CONCLUSIONS: The results revealed that the crude methanolic extract, methanol and ethyl acetate fraction produces remarkable wound healing property due to their antimicrobial and antioxidant activities by possessing the active compounds such as flavonoids, terpenes, alkaloids and saponins.