Natural products are used as traditional medicines from ancient times. From ancient times human beings depended on plants for their food and health purpose. A wide range of plants posses medicinal properties, different parts of plants such as root, stem, flower, fruit, or whole plants are used to cure various health calamities. But the infectious diseases caused by microbes remains a major confront for science even today. It is necessary to have ideal anti-microbial products from plant products because they are considered safer and economic. One such medicinal plant species is Ipomoea reniformis, which belongs to convolvulaceae family and Lamiidae subclass. Its antimicrobial activity was evaluated with aqueous, benzene, ethyl acetate, chloroform and ethanolic extract. The investigation was carried out against various gram positive and gram negative bacterial strains (Escherichia coli NCIM 2109; Staphylococcus aureus NCIM 2079, Pseudomonas aeruginosa NCIM 2036; Bacillus subtilis NCIM 2250). Antifungal activity was carried on (Aspergillus niger NCIM 545). Well diffusion method was employed for the detection of antimicrobial activity. Streptomycin and Amphoterecin B were used as standard. The aqueous extract exhibited activity against Staphylococcus aureus, while the chloroform & ethanolic extract elucidated antifungal activity against Aspergillus niger.