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Development and Validation of difference pectrophotometric method for the estimation of Famotidine in bulk and Pharmaceutical dosage Form

A simple, accurate, precise, reproducible and an economical difference spectrophotometric method has been developed for the estimation of famotidine in bulk and pharmaceutical dosage form. This spectrophotometric method is based on the principle that famotidine shows two different forms in acidic and basic medium that differ in the absorption spectra in acidic and basic medium. The maxima and minima in the difference spectra of famotidine were found to be at 290.60 nm and 259 nm in basic and acidic medium, respectively. The method obeys Beer-Lambert’s law in the concentration range of 5 - 30 μg/ml (r2 = 0.9995). The proposed method was validated as per ICH Q2 (R1) analytical method validation guidelines. The proposed method is suitable for the routine quality control analysis of famotidine in pharmaceutical dosage form

Author(s): Yogita B. Wani, Dipak D. Patil, N. S. Joshi, S. B. Bari

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