This paper describes the development and validation of the HPLC method (254nm) for the quantitation of lopinavir and ritonavir in pure form and pharmaceutical formulations. The method showed to be linear (r2 > 0.999), precise (R.S.D. < 0.21%), accurate (recovery of 100.11% for lopinavir and 99.96% for ritonavir), specific and robust. Three batches of lopinavir and ritonavir tablets were assayed by the validated method. The lopinavir contents in the tablets varied from 99.87 to 100.52%, while ritonavir contents showed 99.61 to 100.57%.