Researchers in nanotechnology are focusing their research towards the development of silver nanomaterial synthesis as they are known to posses inhibitory and bactericidal effects. Resistance towards bacterial infections has emerged in recent years and is a major health hazardous. In the present work attempts were made for the biosynthesis of silver nanoparticles employing the bacteria B. subtilis (MTCC 441). The presence of silver nanoparticles was confirmed using sophisticated techniques such as UV–vis spectrophotometer, size and potential of the synthesized silver nanoparticle was measured quantitatively using Zetasizer. The morphology and uniformity of the particle was determined using Transmission Electron Microscope (TEM). The Protein and silver nanoparticles interaction was identified using Fourier Transform Infrared Spectroscopy (FTIR). Synthesized nanoparticle was also evaluated for its antimicrobial efficacy against S.aureus, P. aeruginosa, E. coli and C .albicans. The highest antimicrobial efficacy was observed for C. albicans and S. aureus. This is the first report on the synthesis and antimicrobial efficacy of silver nanoparticle developed from B .subtilis (MTCC 441).