Biological Synthesis of Zinc oxide Nanoparticles from Catharanthus roseus (l.) G. Don. Leaf extract and validation for antibacterial activity

Biologically synthesized nanoparticles have been widely using in the field of medicine. Research in nanotechnology highlights the possibility of green chemistry pathways to produce technologically important nanomaterials. Present study focuses on the Biological synthesis of Zinc oxide nanoparticles (ZnO-NPs) by Zinc acetate and sodium hydroxide utilizing the biocomponents of leaves of Catharanthus roseus. The samples were characterized by x-ray diffraction (XRD), Scanning Electron Microscopy (SEM), Energy Dispersive Spectroscopy (EDAX) and FT-Raman Spectroscopy. The synthesized ZnO-NPs were found to be spherical in shape with an average size of 23 to 57 nm. These ZnO-NPs were evaluated for antibacterial activity. The maximum diameter of inhibition zones around the ZnONPs disk used for Bacillus thuringiensis indicates the resistance to ZnO NPs followed by Escherichia coli. Among the four bacterial species tested, the Pseudomonas aeuroginosa is more susceptible when compared with other three species. It is concluded that the biological synthesis of ZnO NPs is very fast, easy, cost effective and eco-friendly and without any side effects and ZnO Nps may be used for the preparation of antibacterial formulations against Pseudomonas aeuroginosa

Author(s): G. Bhumi, N. Savithramma

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