In the 1987, Gao and Huang jointly published a very special method and created the name-the “Solid core liposomes with encapsulated colloidal gold particles”. In 1991, Gao et al. studied phagocytosis of protein coated colloidal-gold-agarose-gelatin microbeads by cultured uterine glandular epithelial and stromal cells. They found that the non-phagocytic type fibroblast cells in the cultural dishes turned to be extremely phagocytic type cells in their experimental condition, i.e., fibroblasts after co-incubated with protein coated nano gold rich agarose microbeads would internalize many microbeads that stuffed the entire cell body with microbeads, which looks like a string of purple grape. Because the electron microscopic image showed that each protein coated nano gold rich agarose microbeads after internalization has got an inverted phase of cell membrane in the process of phagocytosis. It is actually a kind of cell-made liposome. The author suggested that if the microbeads were first introduced a toxin in its core and then use crosslinking agent to coat the toxin loaded beads with a specific antibody against certain cancer cells, a novel kind of cell-made targeting drug can be produced.
Johnson K Gao