Objective: To evaluate the antihyperglycemic activity of hydro-alcoholic extract of aerial parts of Mirabilis jalapa L. for its purported use in diabetes. Methods: The study was undertaken by using hydroalcoholic extract of aerial parts of the plant Mirabilis jalapa L.at dose levels of 200 mg/kg and 400 mg/kg (as test) p.o. and standard drug as Metformin (300 mg/kg) in both normoglycemic and streptozotocin induced hyperglycemic animals. Antidiabetic potential was assessed by the measurement of fasting blood glucose, Serum Lipid Profile (total cholesterol, triglycerides), physical parameters (body weight, food and water intake) and Oral Glucose tolerance test. Results: The fasting blood glucose level in normoglycemic, hyperglycemic and in oral glucose tolerance test, showed a significant (p<0.05) decrease at defined time points, while the observed biochemical and physical parameters showed a good agreement with antihyperglycemic property of the extract. Conclusion: The hydroalcoholic extract of aerial parts of Mirabilis jalapa L. possess antihyperglycemic activity which may be mediated through pancreatic and expancreatic pathways, hence justify the folkloric use.