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Abstract

Phytochemical Screening and in Vitro Antimicrobial Investigation of the Methanolic Extract of Xanthium strumarium Leaf

Extract in organic solvent methanol of X. strumarium evaluated for their Phytochemical screening and antibacterial activities against eight pathogenic bacteria such as, Klebsiella pneumoniae NCIM 2719, Proteus vulgaris NCTC 8313, Pseudomonas aeruginosa ATCC 27853, Pseudomonas putida ATCC 12842, Salmonella typhimurium ATCC 23564, Bacillus cereus ATCC 12842, Bacillus subtilis ATCC 6633, Staphylococcus epidermidis ATCC 12228. It was carried out by taking the Methanolic extracts of leaf at a concentration of 50 mg/ml and100 mg/ml their activities were recorded by estimating zones of inhibition as produced by disc-diffusion method on Mueller-Hinton agar media. Based on the activity and preliminary phytochemical analysis TLC and bioautography with different solvent systems resoluted for compounds at different RF valued simultaneously TLC- bio-autography was performed to determine active compounds RF based on TLC.


Author(s): P. Srinivas1 V. Rajashekar2, Upender Rao. E, enkateshwarulu. L and Anil kumar CH

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