The object of the present study was to prepare and evaluate the controlled release of Lamivudine microspheres by W/O/W multiple emulsion solvent evaporation technique using osmogen like sodium chloride, polymers like ethyl cellulose, cellulose acetate and polyvinyl alcohol as a continuous phase. The prepared microspheres were characterized for the percent drug content, encapsulation efficiency, FTIR, scanning electron microscopy (SEM), In vitro dissolution studies, in vitro kinetic studies and accelerated stability studies. FTIR studies showed the stable character of Lamivudine in the microspheres. SEM revealed that the microspheres were smooth and spherical in nature. The release kinetics study revealed that the prepared microspheres were best fitted to the zero order, Higuchi model, Hixson Crowell model and Modified cube root equation for the best formulation. The release kinetics data and characterization studies indicate that drug release from microspheres was diffusion-controlled and that the microspheres were stable.