The obtained pure drug Mesalamine were identified by the FT-IR studies and the particle size were determined. The drug polymer compatibility studies were determined by using FT-IR study and found out no interaction between the drug and polymers. Colon targeted drug delivery system of Mesalamine tablets were prepared by using different formulae by wet and dry granuation method, the successful batches were determined. All the prepared granules were evaluated for the flow properties by studied the angle of repose, compressibility index and Hausners ratio. The prepared granules were studied for the particle size determination by using Malvern particle size analyser. The prepared tablets were evaluated the hardness, friability, weight variation and disintegration studies. Drug content of the formulation were determined by using UPLC instrument. The prepared tablets were coated and the optimized coating formulae were analyzed. The enteric coated tablets were analysed for the disintegration, dissolution and drug content. The stability studies were conducted for the successful batches (F01 &F02) and analysed using UPLC.