In this study, Schiff base ligands were synthesized, characterized and investigated of antibacterial activities on five bacteria. 2-((4-benzyloxy-benzylidene)amino)phenol derived from 4-benzyloxybenzaldehyde and 2- aminophenol. (4-benzyloxy-benzylidene)pyridine-2-yl-amine derived from 4-benzyloxy-benzaldehyde and 2- aminopyridine. The Schiff bases were characterized by elemental analysis, FT-IR and 1H-NMR. The Schiff bases were screened for in-vitro antibacterial activities by disc diffusion and broth microdilution techniques. The minimum inhibitory concentration (MIC) values were calculated by microplate reader at 620 nm. It was found compounds showed mild to moderate antibacterial activity by disc diffusion technique.