The aim of the present study was investigated the improvement of antioxidant enzymes activities and blood brain barrier by Immunox V drug in cerebral spinal fluid of meningitis induced rat model. Twenty four albino rats were selected and divided into three groups of eight; Control group (n=8), Infected group (n=8) and Immunox V treated group (n=8). Our results showed that there were significantly (p<0.001) decreased in all antioxidant enzymes activities along with increased the activities of adenylate kinase, xanthine oxidase enzymes (p<0.001) as well as malonaldialdehyde (MDA) levels in the CSF of infected group as compared to control group. The levels of total protein, calcium and phosphorus were also increased significantly (p<0.001) along with decreased (p<0.001) the glucose level in CSF of infected group as compared to control group. After administration of immunox V for one week treatment, all enzyme activities along with MDA levels as well as biochemical parameters were improved and come back to normal level in the infected plus treated group. These findings indicated that immunox V drug play a therapeutic role in the improvement of oxidant and antioxidant levels and prevent the blood brain barrier during bacterial meningitis infection.