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Protective effect of Chufa Tubers (Cyperus esculentus ) on induction of sperm abnormalities In mice treated with lead acetate

Mahera N Al-Shaikh1; Tala. A. L. Abdul Wahab2; Sahar H Abdul Kareem2; Salim R Hamoudi3
  1. Prof. Dr. Head of Medical Biology, Department of Anatomy, Medical College, University of Baghdad
  2. Lecturer, Department of Basic Science, College of Dentistry, University of Baghdad
  3. Prof. Dr. Head of Department of Pathology, Medical College, University of Baghdad
Corresponding Author: Mahera Noori Al-Shaikh Iraq – Baghdad – College of Medicine – Bab Al- Mouatham E-mail: maheraalshaikh@yahoo.com
Date of Submission: 22-04-2013 Date of Acceptance: 30-05-2013 Conflict of Interest: NIL Source of Support: NONE
Citation: Mahera N Al-Shaikh, Tala. A. L. Abdul Wahab, Sahar H Abdul Kareem, Salim R Hamoudi “Protective effect of Chufa Tubers (Cyperus esculentus ) on induction of sperm abnormalities In mice treated with lead acetate” Int. J. Drug Dev. & Res., April-June 2013, 5(2): 387-392. doi: doi number
Copyright: © 2013 IJDDR, Mahera N Al- Shaikh et al. This is an open access paper distributed under the copyright agreement with Serials Publication, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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Abstract

The effect of the extract of Cyperus esculentus on the changes in sperm count, activity, morphology and testicular histology induces by lead acetate was studied.18 adult male rats were randomly divided into three experimental groups. Group 1 (control) were given clean drinking water and rat chow, group 2 were given intraperitoneally injection 8mg/kg body weight + water + rat chow. Group 3 were given intraperitoneally injection 8mg/kg body weight + water with extract of Cyperus esculentu+ rat cho . The results show a destruction in the testicular histology, decreased sperm count, activity and morphology of Group 2 administered with lead acetate only, which was statistically significant (P<0.05), and there was also a dose-dependent improvement in the parameters studied in the Group 3 given extract of Cyperus esculentus. This shows that the extract of Cyperus esculentus has a regenerative effect on the destroyed testicular histology induced by lead acetate.

INTRODUCTION

Lead is a heavy soft metal, occurs in nature as an oxide or salts .Due to the industrial processes and smokes from petrol vehicle (1) lead is considered as one of the major environmental pollutants and industrial pollution (2, 3). Lead acetate is used as water repellant for mildew protection and as a mordant for cotton dyes. Occupational lead exposure may occur during the manufacture of batteries, painting, printing, pottery glazing, and lead smelting processes .all sources of lead contribute to an increased in permissible exposure limit for metallic lead, lead oxide, and lead salts and soaps that has set by WHO and other health organizations (4).
Lead accumulates virtually in every tissue of the body and effects almost all the body systems specially RBCs, liver, nervous system, gonads and kidneys. Blood lead level is the most reliable indicator of lead intoxication. Neurotoxicity and cognitive loss is a well known side effect of lead even at low does. The oxidative stress of lead causes damage to all components of the cell including cell membrane, proteins and nucleic acids (5).
Literature survey so far available indicates that lead treatment causes anemia (6). Testicular histochemical changes and spermatogenic inhibition have also been observed after lead administration in rats (7). The use of herbs in the treatment of different diseases is fast becoming revolutionalized. In some countries, it has been integrated into the health scheme despite advances in orthodox medicine. It is believed that natural products if utilized in the correct form and dosage are less harmful than synthetic products, which most often elicit some anaphylactic response or reaction (8).
Cyperus esculentus
Cyperus esculentus (or chufa sedge, nut grass, yellow nutsedge, tigernut sedge, or earth almond) is a crop of the family (Cyperaceae) native to warm temperate to subtropical regions of the Northern Hemisphere .The tubers are edible, with a slightly sweet, nutty flavour, compared to the more bitter-tasting tuber of the related Cyperus rotundus (purple nutsedge). They are quite hard and are generally soaked in water before they can be eaten, thus making them much softer and giving them a better texture (9).
In ayurvedic medicine tigernuts are used in the treatment of flatulence, diarrhoea, dysentery, debility and indigestion.[10] Tigernut oil can be used in the cosmetic industry. As it is antidioxide (because of its high content in vitamin E) it helps slow down the ageing of the body cells. It favours the elasticity of the skin and reduces skin wrinkles.[11].Therefore, this study is designed to confirm the effect of lead acetate on the histology of the testes and a possible testicular regenerative effect of Cyperus esculentus Extract.

MATERIALS AND METHODS

The present study was conducted with 20 mature male albino mice, weighing about 25-35 gm was isolated in a relatively controlled environmental temperature of about 25 °C. in the animal breeding center / college of medicine / university f Baghdad. Food & drinking water were available ad labium , 8 mg of Lead acetate (12) dissolved in 1 mL of distilled water was administered in a single intraperitoneal injection to adult mice. 18 animals divided into 3 groups: Group I control group received single intraperitoneally normal saline only.Group II received one injection intraperitoneally of lead acetate (8 mg) only, while group III received lead acetate and herbs extract of Cyprus esculentus. Cyprus esculentus seed were purchased from a local market in Syria. It was rinsed to remove sand and other debris. Soak two tablespoons of cyperus for the whole night in the water, then mashed and filtered (13), drinking with water for period of 4 weeks. Animals were killed on 15th and 30 days of treatment and the testes weighed, testes were dissected out for histopathological study.
The epididymes were excised and minced with fine scissors in physiological saline in a Petri dish. Smears were made on clean, grease free slides .The slides were air-dried and coded for subsequent examination under microscope for morphological abnormalities according to (14). Champers of haemocytometer was charged with 5μI of the fluid, then the sperms were counted and the total numbers of sperms per mm3 were calculated. The data was expressed as Mean ± SD. The differences between groups were compared for statistical significance by t-test with the level of significance set at P≤ 0.05.

Results

In contrast, in the lead acetate treated group, the tests showed an abnormal structure of the seminiferous tubules with vacuolar degenerative changes appearing in the cytoplasm of the spermatogenic epithelium and absence of late stage germ cells showed damage and sloughing of somniferous tubules (Fig. B).Testicular section from animal received lead acetate and treated with herbal extract showed a slight change from normal histological features, somniferous tubules with maturation of spermatogony and sperms inside the lumen (Fig. C ).
Testicular weight and sperm count was significantly decreased in experimental groups compared with normal control group (table I).
Light microscopy was used for evaluation of testicular histology and the seminiferous tubules of the control and different experimental groups shows: control group demonstrated normal somniferous tubules with presence of spermatogony maturation, spermatogenesis was evident in most tubules, and leydic cells are normal (fig. A).
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The results of sperm head abnormality counts showed that there was a high level of abnormality in the sperm head of mice treated with lead acetate compared with control (A). These abnormalities observed are : hook at wrong angle (B ) ,spiral head ( C) , pin-head ( D) , two tails ( E ) , head only (F ) , sperm with no hook (F ) , banana-shaped head ( D) , amorphous head ( D, H ) , mean bent hook (G ) .
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DISCUSSION

Lead (Pb+2) is a heavy metal that can be toxic when introduced into the human and animal bodies by ingestion or inhalation in sufficient quantities. It causes various destructive effects (15). In human, increased levels of lead causes many serious diseases and dysfunction of organs (16).Lead is well known to produce oxidative damage by enhancing lipid peroxidation (17) Lipid peroxidation inactivates cell constituents by oxidation or causes oxidative stress by undergoing radical chain reaction, ultimately leading to loss of membrane integrity (18). In analogy, lead was shown to implicate testicular lipid peroxidation as evidenced by previous reports (19) this could be due to the formation of free radicals (18) through an exhaustion of antioxidants (20) and subsequently to oxidative stress (21). ). On the other hand, since lead does not undergo oxidationreduction cycle, the effect of lead on lipid peroxidation is not a direct effect, but these changes could rather be due to an indirect effect of lead on the free-radical scavenging enzymes( 22) and/or glutathione peroxidase (20). However, the higher membrane lipid content of testes is presumed to make them more vulnerable to oxidative stress (23).
The elevation of frequency of sperm head abnormalities in mice treated with lead acetate may have caused damage to the pre-meiotic stages of spermatogenesis since during spermatogenesis, DNA synthesis occurs before pre-meiotic phase and no further DNA synthesis occurs throughout spermatogenesis in the cell cycle (24, 25).Several reasons have been put forward to explain the increase in the frequency of occurrence of sperm head abnormalities in organisms exposed to some chemicals. In general, damage to the sperm cell is said to occur either by physiological, cytotoxic or genetic mechanism. Odeigah (24) reported that exposure to the chemicals could produce pituitary hypothalamic or sex hormonal effects which in turn could affect spermatogenesis or exposure could cause abnormalities in seminal fluid resulting in functional or structural impairment of sperm . It may also arise as a consequence of naturally occurring level of mistakes in the spermatozoon differentiating process during spermatogenesis (26). In this study, Treated with Cyperus esculentus relatively improves the induced histopathological changes in the testis of rats caused by effect of lead acetate, these improvement may be either : directly on spermatogenesis by reducing lipid peroxidation and prevent or decrease the formation of free radicals , by acting as antioxidant . Or indirectly through pituitary hypothalamic or sex hormonal effects.

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