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IN VITRO EVALUATION OF ANTIOXIDANT POTENTIAL OF VARIOUS EXTRACTS OF WHOLE PLANT OF Bridelia Scandens (Roxb) Willd.

 

Abstract

The antioxidant activities of various extracts of whole plant of Bridelia scandens (Roxb) Willd, was investigated in different in-vitro methods. The antioxidant activity was evaluated by Total antioxidant activity (Phosphomolybdic acid method), FRAP assay with reference standard Ascorbate and total flavonoids content respectively. The methanolic extract of Bridelia scandens was the most effective total antioxidant activity among the extracts. The IC50 values of the methanolic extract of Bridelia scandens and ascorbate was found to be 100µg/ml and 410µg/ml respectively. The methanolic extract of Bridelia scandens was found more effective in FRAP assay than that of petroleum ether and ethyl acetate extracts. But when compare to the all the three extracts with ascorbate (standard), the methanolic extract of the Bridelia scandens showed the significant result. The methanolic extract of Bridelia scandens contains high amount of flavonoids than that of other two extracts. Moreover, the results were observed in a concentration dependent manner. So, the in-vitro studies clearly showed that the methanolic extract of Bridelia scandens has a significant antioxidant activity. These invitro assays indicate that this plant extracts is a significant source of natural antioxidant, which might be helpful in preventing the progress of various oxidative stresses.

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