The objective of the present study was to evaluate the sub-acute toxicity of paracetamol infusion in Mus musculus mice (male and female) at different dose levels, ranging from 16 to 66 mg/kg body weight. There were no major changes in physiological, hematological and biochemical parameters at any dose level used in this study. No mortality was observed in any of the treatment groups during the course of whole study. It has been concluded from the present study that paracetamol infusion is safe even at higher dose. Overall safety and tolerability profile of paracetamol infusion has been proved good and does not appear to carry risk of serious adverse effects in Mus musculus mice.