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RP-HPLC Method Development and Validation of Zaltoprofen in Pure Form and in Pharmaceutical Formulation

A simple, rapid, sensitive and precise RP-HPLC method has been developed for the estimation of zaltoprofen in tablet dosage form. In this method Phenomenax Luna C18, 250 x 4.6 mm, 5mm column with mobile phase consisting of acetonitrile and 0.02 M phosphate buffer in the ratio of 50:50 v/v in isocratic mode was used. The detection wavelength is 232 nm and the flow rate is 1.2 mL/min. The method is linear in the concentration range of 20-120 μg/mL. The linearity of zaltoprofen shows a correlation coefficient of 0.999. The percentage recovery ranges from 99.82-100.46 %. The proposed method was validated by determining linearity, accuracy, precision, specificity, LOD and LOQ.

Author(s): Boovizhikannan Thangabalan, and Palanirajan Vijayaraj Kumar

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