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Abstract

Methylytransferase Activity of Dengue RNA virus by Using Purified Protein

Dengue disease (DENV) is the purpose behind dengue fever. It is a mosquito-borne single positive-stranded RNA contamination of the family Flaviviridae; sort Flavivirus. Dengue illness is a mosquito-borne making or re-rising pathogen. Its positive sense RNA genome has a top at the 5'- end and no poly(A) tail at the 3'- end. The viral RNA encodes a single polyprotein, C-M-E-NS1-NS2A-NS2B-NS3-NS4A-NS4B-NS5. The polyprotein is prepared into three auxiliary proteins (C, M, and E) and seven nonstructural (NS) proteins (NS1, NS2A, NS2B, NS3, NS4A, NS4B, NS5). NS3 and NS5 are multifunctional chemicals performing different undertakings in the viral life cycle. The N-terminal area of NS5 has unmistakable GTP and S-adenosylmethionine (SAM) restricting locales. The job of GTP restricting site is trapped in the guanylyltransferase (GTase) movement of NS5. We depict the development of the MTase space of NS5 in an E. coli articulation vector and conditions for enzymatic activity of N7-and 2'O-methyltransferase exercises that yield the last sort I 5'- topped RNA.


Author(s):

Afaf S. Alwabli*, Sana G. Alattas, Alawiah M. Alhebshi, Nidal M. Zabermawi , Naser Alkenani, Khalid Al-ghmady and Ishtiaq Qadri



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