Genetic alteration occurred in human being due to the exposure various hazardous substances which are leads to array of physiological, biochemical and pathological changes such as enzyme activation, release of mediators, tissue breakdown and inflammation. Turbinaria conoides, marine brown algae commonly found in the Gulf of Mannar, Southeast coast of Tamil Nadu, India was evaluated for its antigenotoxic and antiinÃ¯Â¬Âammatory potential in mice and rat model. Known genotoxin, 4-NQO was used to induce micronuclei in mice bone marrow cells and estimated by micronuclei assay. Acute inflammatory study was carried out with carrageenan induced paw edema in rats, acetic acid induced writhing and tail immersion methods in mice. 4-NQO enhanced the frequency of MnPCEs was about 4.1-fold over the control value, 16.0 ± 0.71 MnPCEs/2500 PCEs. Ethyl acetate fraction of T. conoides along with 4-NQO treated group significantly reduced the frequency of MnPCEs ranging from about 52 to 18%. The reduction of MnPCEs by T. conoides was greatest at 450 mg/kg bw (about 72%) and lowest at 75 mg/kg bw (about 20%). The increased paw edema in rat was measured at time interval of 1, 2, 4 and 8 hours. In both indomethacin and ethyl acetate fraction of T. conoides were observed to be decrease the paw volume significantly based on the dose along with time interval. Hence, writhes and tail immersion methods were also found to be dose dependent and their effective dose (450 mg/kg) observed to be comparable to aspirin standard.
Arumugam P, Murugan M, Ramar M and Murugan K
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